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dc.contributor.authorPanagiotidou, Elpiniki
dc.contributor.authorKonidaris, Constantinos
dc.contributor.authorBaklavaridis, Apostolos
dc.contributor.authorZuburtikudis, Ioannis
dc.contributor.authorAchilias, Dimitris S.
dc.contributor.authorMitlianga, Paraskevi
dc.descriptionPanagiotidou, E., Konidaris, C., Baklavaridis, A., Zuburtikudis, I., Achilias, D., & Mitlianga, P. (2014). A simple route for purifying extracellular poly (3-hydroxybutyrate)-depolymerase from Penicillium pinophilum. Enzyme research, 2014.
dc.description.abstractThis work proposes the purification of an active and efficient enzyme, extracellular poly(3-hydroxybutyrate) (PHB)-depolymerase, suitable for industrial applications. This is achieved by the application of an easy, fast, and cheap route, skipping the chromatography step. Chromatography with one or two columns is a common step in the purification procedure, which however renders the isolation of the enzyme a time consuming and an expensive process. A strain of the fungus Penicillium pinophilum (ATCC 9644) is used for the isolation of extracellular PHB-depolymerase. The molecular weight of the purified enzyme is about 35 kDa and is estimated by gel electrophoresis (SDS-PAGE, 12% polyacrylamide). The enzymatic activity of the isolated enzyme is determined to be 3.56-fold similar to that found by other researchers that have used chromatography for the isolation. The as-isolated enzyme disintegrates the poly(3-hydroxybutyrate) (PHB) films successfully, as it is demonstrated by the biodegradation test results provided here.en_US
dc.titleA Simple Route for Purifying Extracellular Poly(3-hydroxybutyrate)-depolymerase from Penicillium pinophilumen_US

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