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First BRET‐based screening assay performed in budding yeast leads to the discovery of CDK5/p25 interaction inhibitors
(Biotechnology Journal, 2011-07)
The protein kinase CDK5 (cyclin-dependent kinase 5) is activated through its association with a cyclin-like protein p35 or p39. In pathological conditions (such as Alzheimer's disease and various other neuropathies), ...
Yeast two-hybrid methods and their applications in drug discovery
(Elsevier, 2012-02)
The yeast two-hybrid (Y2H) method was first described over 20 years ago. It soon appeared as a major methodological breakthrough in the discovery and analysis of protein interactions, which play a pivotal role in all ...
Erythroid cell mitochondria receive endosomal iron by a “kiss-and-run” mechanism
(Elsevier, 2016-12)
In erythroid cells, more than 90% of transferrin-derived iron enters mitochondria where ferrochelatase inserts Fe2 + into protoporphyrin IX. However, the path of iron from endosomes to mitochondrial ferrochelatase remains ...
A small molecule screen in yeast identifies inhibitors targeting protein–protein interactions within the vaccinia virus replication complex
(Elsevier, 2012-11)
Genetic and biochemical data have identified at least four viral proteins essential for vaccinia virus (VACV) DNA synthesis: the DNA polymerase E9, its processivity factor (the heterodimer A20/D4) and the primase/helicase ...
Inhibition of heme oxygenase ameliorates anemia and reduces iron overload in a β-thalassemia mouse model
(American Society of Hematology, 2018-01)
Thalassemias are a heterogeneous group of red blood cell disorders, considered a major cause of morbidity and mortality among genetic diseases. However, there is still no universally available cure for thalassemias. The ...
The phytochemical and bioactivity profiles of wild Asparagus albus L. plant
(Elsevier, 2017-09)
The ethanolic extracts from the leaves, pericarps and rhizomes of Asparagus albus L. were investigated for their phytochemical composition, antioxidant (DPPH and FRAP assays), anti-microbial against human pathogenic isolates ...
Nrf2 controls iron homoeostasis in haemochromatosis and thalassaemia via Bmp6 and hepcidin
(Nature Publishing Group, 2019-06)
Iron is critical for life but toxic in excess because of iron-catalysed formation of pro-oxidants that cause tissue damage in a range of disorders. The transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2) ...
Antioxidant and hepatoprotective effects of Asparagus albus leaves in carbon tetrachloride‐induced liver injury rats
(Wiley Online Library, 2018-02)
The antioxidant and hepatoprotective activities of hot aqueous extract from Asparagus albus (A. albus) leaf against CCl4-induced liver damage in rats were investigated. The total phenolic, flavonoid, and condensed tannin ...
Pathophysiology and treatment of beta-thalassemia: Investigations of heme oxygenase 1 and its inhibitors
(American Society of Hematology, 2015-12)
Thalassemias are a heterogeneous group of red blood cell disorders ranging from a clinically severe phenotype requiring life-saving transfusions (thalassemia major) to a relatively moderate symptomatic disorder, sometimes ...
Transcriptional induction of transferrin receptors by heme in erythroid cells
(American Society of Hematology, 2015-12)
The transferrin receptor (TfR) is a membrane glycoprotein whose only clearly defined function is to mediate cellular uptake of iron (Fe) from a plasma glycoprotein, transferrin. Iron uptake from diferric transferrin (Tf) ...